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Customized Result Analysis

The following steps will guide you through the analysis of customized microarray or any other high-throughput results.
You have to supply your probes/genes as well as probe/gene annotation file. Up to 500000 probeIDs/geneIDs are allowed in a single analysis. Up to 128 MB annotation file is accepted.

Step one: Upload your customized probe/gene annotation file

Upload your probe/gene annotation file of Gene Onntology (GO) Terms below.
Your annotation file must be of this format.

Maximum file size is 128 MB.
You only have to annotate your probes/genes to directly associated GO terms, GOEAST will automatically expand your annotation to all ancestral GO terms.

Step two: Upload your probe/gene list of interest

Cut and paste your probeIDs/geneIDs below.

Or alternatively, upload a file containing your probeIDs/geneIDs (Up to 10 MB).

Text file is required. Each line should have only one probeID/geneID.

Step Optional: Advanced Parameter Settings

Use default Let me choose

Select statistical test method		Hypergeometric (recommended)		Fisher Exact Test		Χ2 Test
Use Adrian Alexa's improved weighted scoring algorithm?		No (recommended)		Yes (More specific but much slower. Only Fisher Test is available.)
Select multi-test adjustment method		Yekutieli (FDR under dependency) (recommended)	Hochberg (FDR)	Bonferroni	Hochberg	Hommel	Not adjust
Significance Level of Enrichment		0.15 0.1 0.05 0.01 0.001 1e-4 1e-5 We recommed 0.1 for FDR value or 0.01 if not adjust
Minimum # of associated genes in reference (m value) for display		1 2 3 4 5 10 We recommed 5
Graphic result format		PDF (recommended, need Adobe Reader)	PostScript	SVG (might need plug-in)	PNG	GIF	JPEG
Select detail information level		Minimum (Only showing GOID)		Normal (Showing GOID and Term definition)		All (Showing GOID, definition and statistics)
Select font size		6 pt 7 pt 8 pt 9 pt 10 pt 11 pt 12 pt
Optimize the graph width:height ratio		Yes (recommended)	No
Reduce graph size by condensing non-significant nodes to points?		No (recommended)	Yes

Step three: Start analysis

Enter your email address below for sending you the finishing page (required):
   
Name your job below (optional):

Start analysis   Reset
This would take you up to 30 min!
(Note: Up to 15 min extra time is needed if using Adrian Alexa's improved score. See "Advanced Parameter Settings" above for details.)
Your result will be kept for 7 days since your last visit!